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β klotho  (R&D Systems)


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    Structured Review

    R&D Systems β klotho
    β Klotho, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+%CE%B1+klotho+klotho/Mouse+Klotho+beta+Antibody/pmc13098676-151-46-47
    Average 93 stars, based on 30 article reviews
    β klotho - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Injection:

    Article Title: Platelet factors are induced by longevity factor klotho and enhance cognition in young and aging mice.
    Article Snippet: .. Mouse α-Klotho (klotho) (R&D, 1819-KL) was diluted in PBS (pH 7.5) and injected subcutaneously (s.c.) at a volume of 10 μl g−1 (adjusted to weight of mouse) at a dose of 10 μg kg−1 4 h before samples were collected for plasma proteomic analysis, mPF4 ELISA and platelet activation assays. mPF4 (PROSPEC, CHM-245) and hPF4 (PROSEPC, CHM-234 and Genscript, Z03026) were diluted in PBS (pH 7.5) and administered i.p. at a volume of 20 μl g−1 (adjusted to weight of mouse) 1 h before each day of training and testing at a dose of 20 μg kg−1. ..

    Article Title: Platelet factors are induced by longevity factor klotho and enhance cognition in young and aging mice
    Article Snippet: .. Mouse α-Klotho (klotho) (R&D, 1819-KL) was diluted in PBS (pH 7.5) and injected subcutaneously (s.c.) at a volume of 10 μl g −1 (adjusted to weight of mouse) at a dose of 10 μg kg −1 4 h before samples were collected for plasma proteomic analysis, mPF4 ELISA and platelet activation assays. mPF4 (PROSPEC, CHM-245) and hPF4 (PROSEPC, CHM-234 and Genscript, Z03026 ) were diluted in PBS (pH 7.5) and administered i.p. at a volume of 20 μl g −1 (adjusted to weight of mouse) 1 h before each day of training and testing at a dose of 20 μg kg −1 . ..

    Clinical Proteomics:

    Article Title: Platelet factors are induced by longevity factor klotho and enhance cognition in young and aging mice.
    Article Snippet: .. Mouse α-Klotho (klotho) (R&D, 1819-KL) was diluted in PBS (pH 7.5) and injected subcutaneously (s.c.) at a volume of 10 μl g−1 (adjusted to weight of mouse) at a dose of 10 μg kg−1 4 h before samples were collected for plasma proteomic analysis, mPF4 ELISA and platelet activation assays. mPF4 (PROSPEC, CHM-245) and hPF4 (PROSEPC, CHM-234 and Genscript, Z03026) were diluted in PBS (pH 7.5) and administered i.p. at a volume of 20 μl g−1 (adjusted to weight of mouse) 1 h before each day of training and testing at a dose of 20 μg kg−1. ..

    Article Title: Platelet factors are induced by longevity factor klotho and enhance cognition in young and aging mice
    Article Snippet: .. Mouse α-Klotho (klotho) (R&D, 1819-KL) was diluted in PBS (pH 7.5) and injected subcutaneously (s.c.) at a volume of 10 μl g −1 (adjusted to weight of mouse) at a dose of 10 μg kg −1 4 h before samples were collected for plasma proteomic analysis, mPF4 ELISA and platelet activation assays. mPF4 (PROSPEC, CHM-245) and hPF4 (PROSEPC, CHM-234 and Genscript, Z03026 ) were diluted in PBS (pH 7.5) and administered i.p. at a volume of 20 μl g −1 (adjusted to weight of mouse) 1 h before each day of training and testing at a dose of 20 μg kg −1 . ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Platelet factors are induced by longevity factor klotho and enhance cognition in young and aging mice.
    Article Snippet: .. Mouse α-Klotho (klotho) (R&D, 1819-KL) was diluted in PBS (pH 7.5) and injected subcutaneously (s.c.) at a volume of 10 μl g−1 (adjusted to weight of mouse) at a dose of 10 μg kg−1 4 h before samples were collected for plasma proteomic analysis, mPF4 ELISA and platelet activation assays. mPF4 (PROSPEC, CHM-245) and hPF4 (PROSEPC, CHM-234 and Genscript, Z03026) were diluted in PBS (pH 7.5) and administered i.p. at a volume of 20 μl g−1 (adjusted to weight of mouse) 1 h before each day of training and testing at a dose of 20 μg kg−1. ..

    Article Title: Platelet factors are induced by longevity factor klotho and enhance cognition in young and aging mice
    Article Snippet: .. Mouse α-Klotho (klotho) (R&D, 1819-KL) was diluted in PBS (pH 7.5) and injected subcutaneously (s.c.) at a volume of 10 μl g −1 (adjusted to weight of mouse) at a dose of 10 μg kg −1 4 h before samples were collected for plasma proteomic analysis, mPF4 ELISA and platelet activation assays. mPF4 (PROSPEC, CHM-245) and hPF4 (PROSEPC, CHM-234 and Genscript, Z03026 ) were diluted in PBS (pH 7.5) and administered i.p. at a volume of 20 μl g −1 (adjusted to weight of mouse) 1 h before each day of training and testing at a dose of 20 μg kg −1 . ..

    Activation Assay:

    Article Title: Platelet factors are induced by longevity factor klotho and enhance cognition in young and aging mice.
    Article Snippet: .. Mouse α-Klotho (klotho) (R&D, 1819-KL) was diluted in PBS (pH 7.5) and injected subcutaneously (s.c.) at a volume of 10 μl g−1 (adjusted to weight of mouse) at a dose of 10 μg kg−1 4 h before samples were collected for plasma proteomic analysis, mPF4 ELISA and platelet activation assays. mPF4 (PROSPEC, CHM-245) and hPF4 (PROSEPC, CHM-234 and Genscript, Z03026) were diluted in PBS (pH 7.5) and administered i.p. at a volume of 20 μl g−1 (adjusted to weight of mouse) 1 h before each day of training and testing at a dose of 20 μg kg−1. ..

    Article Title: Platelet factors are induced by longevity factor klotho and enhance cognition in young and aging mice
    Article Snippet: .. Mouse α-Klotho (klotho) (R&D, 1819-KL) was diluted in PBS (pH 7.5) and injected subcutaneously (s.c.) at a volume of 10 μl g −1 (adjusted to weight of mouse) at a dose of 10 μg kg −1 4 h before samples were collected for plasma proteomic analysis, mPF4 ELISA and platelet activation assays. mPF4 (PROSPEC, CHM-245) and hPF4 (PROSEPC, CHM-234 and Genscript, Z03026 ) were diluted in PBS (pH 7.5) and administered i.p. at a volume of 20 μl g −1 (adjusted to weight of mouse) 1 h before each day of training and testing at a dose of 20 μg kg −1 . ..



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    Image Search Results


    α-Klotho treatment protects against exercise-induced fatigue. ( a ) Changes in mice bodyweight during 6-day exercise training ( n = 10 per group), ( b ) Mice bodyweight before sacrifice (day 7) ( n = 10 per group), ( c ) Grip strength of mice ( n = 10 per group), ( d ) Grip strength normalized by bodyweight, ( e ) Changes of time to exhaustion during exercise training ( n = 10 per group), changes of blood CK ( f ), BUN ( g ), and lactic acid ( h ) among groups ( n = 10 per group). Data represented as mean ± SD. * p < 0.05, ** p < 0.01; α-Klotho vs. Control, # p < 0.05; Saline vs. Control, & p < 0.05; α-Klotho vs. Saline, $ p < 0.05.

    Journal: International Journal of Molecular Sciences

    Article Title: α-Klotho Supplementation Mitigates Cumulative Exercise-Induced Fatigue via Coordinated NRF2-Mediated Antioxidant Defense and AKT/GS-Driven Hepatic Glycogen Supercompensation in Mice

    doi: 10.3390/ijms27010412

    Figure Lengend Snippet: α-Klotho treatment protects against exercise-induced fatigue. ( a ) Changes in mice bodyweight during 6-day exercise training ( n = 10 per group), ( b ) Mice bodyweight before sacrifice (day 7) ( n = 10 per group), ( c ) Grip strength of mice ( n = 10 per group), ( d ) Grip strength normalized by bodyweight, ( e ) Changes of time to exhaustion during exercise training ( n = 10 per group), changes of blood CK ( f ), BUN ( g ), and lactic acid ( h ) among groups ( n = 10 per group). Data represented as mean ± SD. * p < 0.05, ** p < 0.01; α-Klotho vs. Control, # p < 0.05; Saline vs. Control, & p < 0.05; α-Klotho vs. Saline, $ p < 0.05.

    Article Snippet: After blocking with 5% non-fat milk, the membranes were incubated at 4 °C overnight with the following primary antibodies (all diluted 1:1000): α-Klotho (R&D Systems, Minneapolis, MN, USA, Cat# AF1819), NRF2 (Proteintech, Wuhan, Hubei, China, Cat # 16396-1-AP), p-NRF2 (Abways, Shanghai, China, Cat # CY6573), HO-1 (GenTex, Irvine, CA, USA, Cat # GTX13248), PGC-1α (Proteintech, Wuhan, Hubei, China, Cat # 66369-1-Ig), p-GS (Proteintech, Wuhan, Hubei, China, Cat # 28855-1-AP), GS (Proteintech, Wuhan, Hubei, China, Cat # 10566-1-AP), GLUT4 (Proteintech, Wuhan, Hubei, China, Cat # 66846-1-Ig), p-AKT (CST, Danvers, MA, USA, Cat # 4060S), t-AKT (CST, Danvers, MA, USA, Cat # 9272S), and GAPDH (CST, Danvers, MA, USA, Cat # 2118S).

    Techniques: Control, Saline

    α-Klotho treatment attenuates oxidative stress in muscle. Changes of H 2 O 2 ( a ), MDA ( b ), SOD ( c ), T-GSH ( d ), GSSG ( e ), GSH ( f ), and GSH/GSSG ( g ) in muscle ( n = 10 per group). Data represented as mean ± SD. * p < 0.05, ** p < 0.01.

    Journal: International Journal of Molecular Sciences

    Article Title: α-Klotho Supplementation Mitigates Cumulative Exercise-Induced Fatigue via Coordinated NRF2-Mediated Antioxidant Defense and AKT/GS-Driven Hepatic Glycogen Supercompensation in Mice

    doi: 10.3390/ijms27010412

    Figure Lengend Snippet: α-Klotho treatment attenuates oxidative stress in muscle. Changes of H 2 O 2 ( a ), MDA ( b ), SOD ( c ), T-GSH ( d ), GSSG ( e ), GSH ( f ), and GSH/GSSG ( g ) in muscle ( n = 10 per group). Data represented as mean ± SD. * p < 0.05, ** p < 0.01.

    Article Snippet: After blocking with 5% non-fat milk, the membranes were incubated at 4 °C overnight with the following primary antibodies (all diluted 1:1000): α-Klotho (R&D Systems, Minneapolis, MN, USA, Cat# AF1819), NRF2 (Proteintech, Wuhan, Hubei, China, Cat # 16396-1-AP), p-NRF2 (Abways, Shanghai, China, Cat # CY6573), HO-1 (GenTex, Irvine, CA, USA, Cat # GTX13248), PGC-1α (Proteintech, Wuhan, Hubei, China, Cat # 66369-1-Ig), p-GS (Proteintech, Wuhan, Hubei, China, Cat # 28855-1-AP), GS (Proteintech, Wuhan, Hubei, China, Cat # 10566-1-AP), GLUT4 (Proteintech, Wuhan, Hubei, China, Cat # 66846-1-Ig), p-AKT (CST, Danvers, MA, USA, Cat # 4060S), t-AKT (CST, Danvers, MA, USA, Cat # 9272S), and GAPDH (CST, Danvers, MA, USA, Cat # 2118S).

    Techniques:

    α-Klotho activates NRF2/HO-1 pathway in muscle. ( a ) Representative western blot band. Expression of α-klotho ( b ), p-NRF2/t-NRF2 ( c ), HO-1 ( d ), and PGC-1α ( e ) in muscle ( n = 6 per group). Data represented as mean ± SD. * p < 0.05, ** p < 0.01.

    Journal: International Journal of Molecular Sciences

    Article Title: α-Klotho Supplementation Mitigates Cumulative Exercise-Induced Fatigue via Coordinated NRF2-Mediated Antioxidant Defense and AKT/GS-Driven Hepatic Glycogen Supercompensation in Mice

    doi: 10.3390/ijms27010412

    Figure Lengend Snippet: α-Klotho activates NRF2/HO-1 pathway in muscle. ( a ) Representative western blot band. Expression of α-klotho ( b ), p-NRF2/t-NRF2 ( c ), HO-1 ( d ), and PGC-1α ( e ) in muscle ( n = 6 per group). Data represented as mean ± SD. * p < 0.05, ** p < 0.01.

    Article Snippet: After blocking with 5% non-fat milk, the membranes were incubated at 4 °C overnight with the following primary antibodies (all diluted 1:1000): α-Klotho (R&D Systems, Minneapolis, MN, USA, Cat# AF1819), NRF2 (Proteintech, Wuhan, Hubei, China, Cat # 16396-1-AP), p-NRF2 (Abways, Shanghai, China, Cat # CY6573), HO-1 (GenTex, Irvine, CA, USA, Cat # GTX13248), PGC-1α (Proteintech, Wuhan, Hubei, China, Cat # 66369-1-Ig), p-GS (Proteintech, Wuhan, Hubei, China, Cat # 28855-1-AP), GS (Proteintech, Wuhan, Hubei, China, Cat # 10566-1-AP), GLUT4 (Proteintech, Wuhan, Hubei, China, Cat # 66846-1-Ig), p-AKT (CST, Danvers, MA, USA, Cat # 4060S), t-AKT (CST, Danvers, MA, USA, Cat # 9272S), and GAPDH (CST, Danvers, MA, USA, Cat # 2118S).

    Techniques: Western Blot, Expressing

    α-Klotho increases liver glycogen content. Glycogen content in muscle ( a ) and the liver ( b ) ( n = 10 per group). Data represented as mean ± SD. ** p < 0.01.

    Journal: International Journal of Molecular Sciences

    Article Title: α-Klotho Supplementation Mitigates Cumulative Exercise-Induced Fatigue via Coordinated NRF2-Mediated Antioxidant Defense and AKT/GS-Driven Hepatic Glycogen Supercompensation in Mice

    doi: 10.3390/ijms27010412

    Figure Lengend Snippet: α-Klotho increases liver glycogen content. Glycogen content in muscle ( a ) and the liver ( b ) ( n = 10 per group). Data represented as mean ± SD. ** p < 0.01.

    Article Snippet: After blocking with 5% non-fat milk, the membranes were incubated at 4 °C overnight with the following primary antibodies (all diluted 1:1000): α-Klotho (R&D Systems, Minneapolis, MN, USA, Cat# AF1819), NRF2 (Proteintech, Wuhan, Hubei, China, Cat # 16396-1-AP), p-NRF2 (Abways, Shanghai, China, Cat # CY6573), HO-1 (GenTex, Irvine, CA, USA, Cat # GTX13248), PGC-1α (Proteintech, Wuhan, Hubei, China, Cat # 66369-1-Ig), p-GS (Proteintech, Wuhan, Hubei, China, Cat # 28855-1-AP), GS (Proteintech, Wuhan, Hubei, China, Cat # 10566-1-AP), GLUT4 (Proteintech, Wuhan, Hubei, China, Cat # 66846-1-Ig), p-AKT (CST, Danvers, MA, USA, Cat # 4060S), t-AKT (CST, Danvers, MA, USA, Cat # 9272S), and GAPDH (CST, Danvers, MA, USA, Cat # 2118S).

    Techniques:

    α-Klotho activates the AKT signaling pathway in the liver. ( a ) Representative western blot band. Expression of α-klotho ( b ), p-GS/t-GS ( c ), t-GS ( d ), p-AKT/t-AKT ( e ), GLUT4 ( f ), and PGC-1α ( g ) in the liver ( n = 6 per group). Data represented as mean ± SD. * p < 0.05, ** p < 0.01.

    Journal: International Journal of Molecular Sciences

    Article Title: α-Klotho Supplementation Mitigates Cumulative Exercise-Induced Fatigue via Coordinated NRF2-Mediated Antioxidant Defense and AKT/GS-Driven Hepatic Glycogen Supercompensation in Mice

    doi: 10.3390/ijms27010412

    Figure Lengend Snippet: α-Klotho activates the AKT signaling pathway in the liver. ( a ) Representative western blot band. Expression of α-klotho ( b ), p-GS/t-GS ( c ), t-GS ( d ), p-AKT/t-AKT ( e ), GLUT4 ( f ), and PGC-1α ( g ) in the liver ( n = 6 per group). Data represented as mean ± SD. * p < 0.05, ** p < 0.01.

    Article Snippet: After blocking with 5% non-fat milk, the membranes were incubated at 4 °C overnight with the following primary antibodies (all diluted 1:1000): α-Klotho (R&D Systems, Minneapolis, MN, USA, Cat# AF1819), NRF2 (Proteintech, Wuhan, Hubei, China, Cat # 16396-1-AP), p-NRF2 (Abways, Shanghai, China, Cat # CY6573), HO-1 (GenTex, Irvine, CA, USA, Cat # GTX13248), PGC-1α (Proteintech, Wuhan, Hubei, China, Cat # 66369-1-Ig), p-GS (Proteintech, Wuhan, Hubei, China, Cat # 28855-1-AP), GS (Proteintech, Wuhan, Hubei, China, Cat # 10566-1-AP), GLUT4 (Proteintech, Wuhan, Hubei, China, Cat # 66846-1-Ig), p-AKT (CST, Danvers, MA, USA, Cat # 4060S), t-AKT (CST, Danvers, MA, USA, Cat # 9272S), and GAPDH (CST, Danvers, MA, USA, Cat # 2118S).

    Techniques: Western Blot, Expressing